› 1vja

X-ray diffraction
2Å resolution

Function and Biology Details

Reaction catalysed: 
Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133249 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Urokinase-type plasminogen activator chain B Chain: U
Molecule details ›
Chain: U
Length: 262 amino acids
Theoretical weight: 29.68 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00749 (Residues: 164-425; Coverage: 64%)
Gene name: PLAU
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand 7IN 1 x 7IN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 53.065Å b: 55.004Å c: 81.924Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 not available 0.232
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Therapeutics targeting the fibrinolytic system.
Lin et al. (2020)
11 more

4 mentions without citation

Urokinase plasminogen activator as an anti-metastasis target: inhibitor design principles, recent amiloride derivatives, and issues with human/mouse species selectivity.
El Salamouni et al. (2022)
3 more