› 1vrc

Solution NMR

Complex of enzyme IIAmannose and the histidine-containing phosphocarrier protein HPr from escherichia coli nmr, restrained regularized mean structure

Released:
DOI: 10.2210/pdb1vrc/pdb
PDB entry 1vrc coloured by chain, ensemble of 2 models, front view.
PDB entry 1vrc coloured by chain, ensemble of 2 models, side view.
PDB entry 1vrc coloured by chain, ensemble of 2 models, top view.
Source organism: Escherichia coli
Primary publication:
Solution NMR structure of the 48-kDa IIAMannose-HPr complex of the Escherichia coli mannose phosphotransferase system.
Williams DC, Cai M, Suh JY, Peterkofsky A, Clore GM
J Biol Chem 280 20775-84 (2005)
PMID: 15788390

Function and Biology Details

Reaction catalysed: 
[Protein]-N(pi)-phospho-L-histidine + D-mannose(Side 1) = [protein]-L-histidine + D-mannose 6-phosphate(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-532446 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
PTS system mannose-specific EIIAB component Chains: A, B
Molecule details ›
Chains: A, B
Length: 136 amino acids
Theoretical weight: 14.76 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P69797 (Residues: 1-133; Coverage: 41%)
Gene names: JW1806, b1817, gptB, manX, ptsL
Sequence domains: PTS system fructose IIA component
Structure domains: Phosphotransferase system, mannose-type IIA component
Phosphocarrier protein HPr Chains: C, D
Molecule details ›
Chains: C, D
Length: 85 amino acids
Theoretical weight: 9.13 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AA04 (Residues: 1-85; Coverage: 100%)
Gene names: JW2408, b2415, hpr, ptsH
Sequence domains: PTS HPr component phosphorylation site
Structure domains: HPr-like

Ligands and Environments

1 bound ligand:
Ligand PO3 2 x PO3
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: CONJOINED RIGID BODY/TORSION ANGLE DYNAMICS
Expression system: Escherichia coli

Quick links

Citations

10 review citations

How phosphotransferase system-related protein phosphorylation regulates carbohydrate metabolism in bacteria.
Deutscher et al. (2006)
9 more

4 mentions without citation

Replica exchange simulations of transient encounter complexes in protein-protein association.
Kim et al. (2008)
3 more