1vyg › Citations

Schistosoma mansoni fatty acid binding protein: specificity and functional control as revealed by crystallographic structure.

Angelucci F, Johnson KA, Baiocco P, Miele AE, Brunori M, Valle C, Vigorosi F, Troiani AR, Liberti P, Cioli D, Klinkert MQ, Bellelli A
Biochemistry 43 13000-11 (2004)
Cited: 18 times
EuropePMC logo PMID: 15476393

Abstract

Schistosoma mansoni fatty acid binding protein (Sm14) was crystallized with bound oleic acid (OLA) and arachidonic acid (ACD), and their structures were solved at 1.85 and 2.4 A resolution, respectively. Sm14 is a vaccine target for schistosomiasis, the second most prevalent parasitic disease in humans. The parasite is unable to synthesize fatty acids depending on the host for these nutrients. Moreover, arachidonic acid (ACD) is required to synthesize prostaglandins employed by schistosomes to evade the host's immune defenses. In the complex, the hydrocarbon tail of bound OLA assumes two conformations, whereas ACD adopts a unique hairpin-looped structure. ACD establishes more specific interactions with the protein, among which the most important is a pi-cation bond between Arg78 and the double bond at C8. Comparison with homologous fatty acid binding proteins suggests that the binding site of Sm14 is optimized to fit ACD. To test the functional implications of our structural data, the affinity of Sm14 for 1,8-anilinonaphthalenesulfonic acid (ANS) has been measured; moreover the binding constants of six different fatty acids were determined from their ability to displace ANS. OLA and ACD exhibited the highest affinities. To determine the rates of fatty acid binding and dissociation we carried out stopped flow kinetic experiments monitoring displacement by (and of) ANS. The binding rate constant of ligands is controlled by a slow pH dependent conformational change, which we propose to have physiological relevance.

Articles - 1vyg mentioned but not cited (4)

  1. The Recognition of Identical Ligands by Unrelated Proteins. Barelier S, Sterling T, O'Meara MJ, Shoichet BK. ACS Chem Biol 10 2772-2784 (2015)
  2. Local error estimates dramatically improve the utility of homology models for solving crystal structures by molecular replacement. Bunkóczi G, Wallner B, Read RJ. Structure 23 397-406 (2015)
  3. Three Dimensional Structure Prediction of Fatty Acid Binding Site on Human Transmembrane Receptor CD36. Tarhda Z, Semlali O, Kettani A, Moussa A, Abumrad NA, Ibrahimi A. Bioinform Biol Insights 7 369-373 (2013)
  4. Schistosoma mansoni venom allergen-like protein 6 (SmVAL6) maintains tegumental barrier function. Perally S, Geyer KK, Farani PSG, Chalmers IW, Fernandez-Fuentes N, Maskell DR, Hulme BJ, Forde-Thomas J, Phillips D, Farias LP, Collins JJ, Hoffmann KF. Int J Parasitol 51 251-261 (2021)


Reviews citing this publication (2)

  1. Current Status of the Sm14/GLA-SE Schistosomiasis Vaccine: Overcoming Barriers and Paradigms towards the First Anti-Parasitic Human(itarian) Vaccine. Tendler M, Almeida MS, Vilar MM, Pinto PM, Limaverde-Sousa G. Trop Med Infect Dis 3 E121 (2018)
  2. The unusual lipid binding proteins of parasitic helminths and their potential roles in parasitism and as therapeutic targets. Franchini GR, Pórfido JL, Ibáñez Shimabukuro M, Rey Burusco MF, Bélgamo JA, Smith BO, Kennedy MW, Córsico B. Prostaglandins Leukot Essent Fatty Acids 93 31-36 (2015)

Articles citing this publication (12)

  1. Structural basis for ligand regulation of the fatty acid-binding protein 5, peroxisome proliferator-activated receptor β/δ (FABP5-PPARβ/δ) signaling pathway. Armstrong EH, Goswami D, Griffin PR, Noy N, Ortlund EA. J Biol Chem 289 14941-14954 (2014)
  2. Two modes of fatty acid binding to bovine β-lactoglobulin--crystallographic and spectroscopic studies. Loch J, Polit A, Górecki A, Bonarek P, Kurpiewska K, Dziedzicka-Wasylewska M, Lewiński K. J Mol Recognit 24 341-349 (2011)
  3. In vitro and in vivo effects of unsaturated fatty acids on Schistosoma mansoni and S. haematobium lung-stage larvae. Tallima H, Salah M, El-Ridi R. J Parasitol 91 1094-1102 (2005)
  4. Comparative analysis of two fatty acid binding proteins from Fasciola gigantica. Chunchob S, Grams R, Viyanant V, Smooker PM, Vichasri-Grams S. Parasitology 137 1805-1817 (2010)
  5. Crystal structure of axolotl (Ambystoma mexicanum) liver bile acid-binding protein bound to cholic and oleic acid. Capaldi S, Guariento M, Perduca M, Di Pietro SM, Santomé JA, Monaco HL. Proteins 64 79-88 (2006)
  6. Lipid binding proteins from parasitic platyhelminthes. Alvite G, Esteves A. Front Physiol 3 363 (2012)
  7. Lipocalin Q83 reveals a dual ligand binding mode with potential implications for the functions of siderocalins. Coudevylle N, Hoetzinger M, Geist L, Kontaxis G, Hartl M, Bister K, Konrat R. Biochemistry 50 9192-9199 (2011)
  8. Structural basis for the ligand-binding specificity of fatty acid-binding proteins (pFABP4 and pFABP5) in gentoo penguin. Lee CW, Kim JE, Do H, Kim RO, Lee SG, Park HH, Chang JH, Yim JH, Park H, Kim IC, Lee JH. Biochem Biophys Res Commun 465 12-18 (2015)
  9. In silico studies of Echinococcus granulosus FABPs. Esteves A, Paulino M. J Biomol Struct Dyn 31 224-239 (2013)
  10. Computer-Assisted Discovery of Alkaloids with Schistosomicidal Activity. Menezes RPB, Viana JO, Muratov E, Scotti L, Scotti MT. Curr Issues Mol Biol 44 383-408 (2022)
  11. Structure and ligand binding of As-p18, an extracellular fatty acid binding protein from the eggs of a parasitic nematode. Ibáñez-Shimabukuro M, Rey-Burusco MF, Gabrielsen M, Franchini GR, Riboldi-Tunnicliffe A, Roe AJ, Griffiths K, Cooper A, Córsico B, Kennedy MW, Smith BO. Biosci Rep 39 BSR20191292 (2019)
  12. Successful detection, expression and purification of the alternatively spliced truncated Sm14 antigen of an Egyptian strain of Schistosoma mansoni. Ewaisha RE, Bahey-El-Din M, Mossallam SF, Khalil AM, Aboushleib HM. J Helminthol 89 764-768 (2015)


Quick links