› 1w10

X-ray diffraction
2Å resolution

Urokinase type plasminogen activator

Released:
DOI: 10.2210/pdb1w10/pdb
PDB entry 1w10 coloured by chain, front view.
PDB entry 1w10 coloured by chain, side view.
PDB entry 1w10 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystals of urokinase type plasminogen activator complexes reveal the binding mode of peptidomimetic inhibitors.
Zeslawska E, Jacob U, Schweinitz A, Coombs G, Bode W, Madison E
J Mol Biol 328 109-18 (2003)
PMID: 12684001

Function and Biology Details

Reaction catalysed: 
Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133249 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Urokinase-type plasminogen activator chain B Chain: U
Molecule details ›
Chain: U
Length: 247 amino acids
Theoretical weight: 27.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00749 (Residues: 179-425; Coverage: 60%)
Gene name: PLAU
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

2 bound ligands:
Ligand SJ1 1 x SJ1
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 53.21Å b: 54.67Å c: 82.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.19 not available
Expression system: Escherichia coli

Quick links

Citations

2 review citations

The plasminogen activation system in periodontal tissue (Review).
Wyganowska-Świątkowska et al. (2014)
1 more

1 mention without citation

Investigation of the Compatibility between Warheads and Peptidomimetic Sequences of Protease Inhibitors-A Comprehensive Reactivity and Selectivity Study.
Müller et al. (2023)