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beta-fructosidase from Thermotoga maritima in complex with raffinose

X-ray diffraction
1.87Å resolution
PDB entry 1w2t coloured by chain, front view.
PDB entry 1w2t coloured by chain, side view.
PDB entry 1w2t coloured by chain, top view.
Source organism: Thermotoga maritima MSB8
Assembly composition:
monomeric (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1
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Assembly Name: Beta-fructosidase
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Multimeric state: monomeric
Accessible surface area: 18068.65 Å2
Buried surface area: 1345.21 Å2
Dissociation area: 144.98 Å2
Dissociation energy (ΔGdiss): -3.21 kcal/mol
Dissociation entropy (TΔSdiss): 2.74 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-128504
Assembly 2
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Assembly Name: Beta-fructosidase
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Multimeric state: monomeric
Accessible surface area: 18042.44 Å2
Buried surface area: 1263.38 Å2
Dissociation area: 414.6 Å2
Dissociation energy (ΔGdiss): -5.41 kcal/mol
Dissociation entropy (TΔSdiss): 5.24 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-128504
Assembly 3 (preferred)
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Assembly Name: Beta-fructosidase
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Multimeric state: monomeric
Accessible surface area: 18333.6 Å2
Buried surface area: 1412.44 Å2
Dissociation area: 412.43 Å2
Dissociation energy (ΔGdiss): -5.74 kcal/mol
Dissociation entropy (TΔSdiss): 5.24 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-128504
Assembly 4
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Assembly Name: Beta-fructosidase
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Multimeric state: monomeric
Accessible surface area: 18162.98 Å2
Buried surface area: 1067.44 Å2
Dissociation area: 415.07 Å2
Dissociation energy (ΔGdiss): -5.71 kcal/mol
Dissociation entropy (TΔSdiss): 5.24 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-128504
Assembly 5
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Assembly Name: Beta-fructosidase
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Multimeric state: monomeric
Accessible surface area: 18227.63 Å2
Buried surface area: 1252.19 Å2
Dissociation area: 413.05 Å2
Dissociation energy (ΔGdiss): -5.68 kcal/mol
Dissociation entropy (TΔSdiss): 5.25 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-128504
Assembly 6
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Assembly Name: Beta-fructosidase
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Multimeric state: monomeric
Accessible surface area: 18001.35 Å2
Buried surface area: 1049.9 Å2
Dissociation area: 406.12 Å2
Dissociation energy (ΔGdiss): -6.97 kcal/mol
Dissociation entropy (TΔSdiss): 5.24 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-128504

Macromolecules

Beta-fructosidase
Chains: A, B, C, D, E, F
Length: 432 amino acids
Theoretical weight: 49.87 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O33833 (Residues: 1-432; Coverage: 100%)
Gene names: TM_1414, bfrA
Pfam:
InterPro:
CATH:
SCOP:
Beta-fructosidase in PDB entry 1w2t, assembly 3, front view.
Beta-fructosidase in PDB entry 1w2t, assembly 3, side view.
Beta-fructosidase in PDB entry 1w2t, assembly 3, top view.
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