› 1w6j

X-ray diffraction
2.2Å resolution

Structure of human OSC in complex with Ro 48-8071

Released:
DOI: 10.2210/pdb1w6j/pdb
PDB entry 1w6j coloured by chain, front view.
PDB entry 1w6j coloured by chain, side view.
PDB entry 1w6j coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Insight into steroid scaffold formation from the structure of human oxidosqualene cyclase.
Thoma R, Schulz-Gasch T, D'Arcy B, Benz J, Aebi J, Dehmlow H, Hennig M, Stihle M, Ruf A
Nature 432 118-22 (2004)
PMID: 15525992

Function and Biology Details

Reaction catalysed: 
(3S)-2,3-epoxy-2,3-dihydrosqualene = lanosterol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155734 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lanosterol synthase Chain: A
Molecule details ›
Chain: A
Length: 732 amino acids
Theoretical weight: 83.41 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P48449 (Residues: 1-732; Coverage: 100%)
Gene names: LSS, OSC
Sequence domains: Prenyltransferase and squalene oxidase repeat
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand R71 1 x R71
Ligand BOG 3 x BOG
Ligand C14 1 x C14
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2221
Unit cell:
a: 189.915Å b: 202.424Å c: 62.638Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.17 0.199
Expression system: Komagataella pastoris

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Citations

32 review citations

Structural and Chemical Biology of Terpenoid Cyclases.
Christianson DW. (2017)
31 more

13 mentions without citation

Applications of protein engineering in the microbial synthesis of plant triterpenoids.
Luo et al. (2023)
12 more