1w7p

X-ray diffraction
3.6Å resolution

The crystal structure of endosomal complex ESCRT-II (VPS22/VPS25/VPS36)

Released:
Model geometry
Fit model/data

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155605 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Vacuolar-sorting protein SNF8 Chain: A
Molecule details ›
Chain: A
Length: 233 amino acids
Theoretical weight: 26.99 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q12483 (Residues: 1-233; Coverage: 100%)
Gene names: SNF8, VPS22, YPL002C
Sequence domains: EAP30/Vps36 family
Structure domains:
Vacuolar protein-sorting-associated protein 25 Chains: B, C
Molecule details ›
Chains: B, C
Length: 202 amino acids
Theoretical weight: 23.58 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P47142 (Residues: 1-202; Coverage: 100%)
Gene names: J1957, VPS25, VPT25, YJR102C
Sequence domains: ESCRT-II complex subunit
Structure domains:
Vacuolar protein-sorting-associated protein 36 Chain: D

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P4322
Unit cell:
a: 149.908Å b: 149.908Å c: 186.152Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.294 0.292 0.33
Expression system: Escherichia coli BL21(DE3)