› 1w9h

X-ray diffraction
1.95Å resolution

The Structure of a Piwi protein from Archaeoglobus fulgidus.

Released:
DOI: 10.2210/pdb1w9h/pdb
PDB entry 1w9h coloured by chain, front view.
PDB entry 1w9h coloured by chain, side view.
PDB entry 1w9h coloured by chain, top view.
Source organism: Archaeoglobus fulgidus
Primary publication:
Crystal structure of a PIWI protein suggests mechanisms for siRNA recognition and slicer activity.
Parker JS, Roe SM, Barford D
EMBO J 23 4727-37 (2004)
PMID: 15565169

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128084 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Piwi protein Chain: A
Molecule details ›
Chain: A
Length: 427 amino acids
Theoretical weight: 49.3 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O28951 (Residues: 1-427; Coverage: 100%)
Gene name: AF_1318
Sequence domains: Piwi domain
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CD 2 x CD
Ligand NI 5 x NI
Ligand CL 4 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P21212
Unit cell:
a: 69.09Å b: 137.863Å c: 51.663Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.192 0.237
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

70 review citations

Origins and Mechanisms of miRNAs and siRNAs.
Carthew et al. (2009)
69 more

15 mentions without citation

A Structural View of miRNA Biogenesis and Function.
Leitão et al. (2022)
14 more