› 1wa4

X-ray diffraction
2.1Å resolution

Crystal structure of the M131F L135A EvaD double mutant

Released:
DOI: 10.2210/pdb1wa4/pdb
PDB entry 1wa4 coloured by chain, front view.
PDB entry 1wa4 coloured by chain, side view.
PDB entry 1wa4 coloured by chain, top view.
Source organism: Amycolatopsis orientalis
Entry authors: Merkel AB, Naismith JH

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129416 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PCZA361.16 Chain: A
Molecule details ›
Chain: A
Length: 205 amino acids
Theoretical weight: 22.49 KDa
Source organism: Amycolatopsis orientalis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O52806 (Residues: 1-205; Coverage: 100%)
Sequence domains: dTDP-4-dehydrorhamnose 3,5-epimerase
Structure domains: Jelly Rolls

Ligands and Environments

1 bound ligand:
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: C2
Unit cell:
a: 51.632Å b: 79.101Å c: 48.777Å
α: 90° β: 98.75° γ: 90°
R-values:
R R work R free
0.18 0.176 0.248
Expression system: Escherichia coli BL21(DE3)

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