› 1wb8

X-ray diffraction
2.3Å resolution

Iron Superoxide Dismutase (FE-SOD) from the Hyperthermophile SULFOLOBUS SOLFATARICUS. 2.3 A Resolution Structure of Recombinant Protein with a Covalently Modified Tyrosine in the Active Site.

Released:
DOI: 10.2210/pdb1wb8/pdb
PDB entry 1wb8 coloured by chain, front view.
PDB entry 1wb8 coloured by chain, side view.
PDB entry 1wb8 coloured by chain, top view.
Source organism: Saccharolobus solfataricus
Primary publication:
Iron superoxide dismutase from the archaeon Sulfolobus solfataricus: analysis of structure and thermostability.
Ursby T, Adinolfi BS, Al-Karadaghi S, De Vendittis E, Bocchini V
J Mol Biol 286 189-205 (1999)
PMID: 9931259

Function and Biology Details

Reaction catalysed: 
2 superoxide + 2 H(+) = O(2) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160455 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Superoxide dismutase [Fe] Chains: A, B
Molecule details ›
Chains: A, B
Length: 210 amino acids
Theoretical weight: 24.14 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: P80857 (Residues: 2-211; Coverage: 100%)
Gene names: SSO0316, sod
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand FE 2 x FE
Ligand PMS 2 x PMS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU ROTAFLEX RU-2
Spacegroup: C2
Unit cell:
a: 76.266Å b: 124.324Å c: 60.268Å
α: 90° β: 128.75° γ: 90°
R-values:
R R work R free
0.167 0.167 0.187
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Hyperthermophilic enzymes: sources, uses, and molecular mechanisms for thermostability.
Vieille et al. (2001)
3 more

4 mentions without citation

Sulfonyl fluorides as privileged warheads in chemical biology.
Narayanan et al. (2015)
3 more