› 1wet

X-ray diffraction
2.6Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114766 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
HTH-type transcriptional repressor PurR Chain: A
Molecule details ›
Chain: A
Length: 340 amino acids
Theoretical weight: 38.09 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0ACP7 (Residues: 2-341; Coverage: 100%)
Gene names: JW1650, b1658, purR
Sequence domains:
Structure domains:
DNA (5'-D(*AP*AP*CP*GP*AP*AP*AP*AP*CP*GP*TP*TP*TP*TP*CP*GP*T )-3') Chain: B
Molecule details ›
Chain: B
Length: 17 nucleotides
Theoretical weight: 5.21 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand GUN 1 x GUN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2221
Unit cell:
a: 175.99Å b: 95.08Å c: 81.51Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 not available not available
Expression system: Not provided

Quick links

Citations

3 review citations

An overview of the structures of protein-DNA complexes.
Luscombe et al. (2000)
2 more

17 mentions without citation

Allostery: absence of a change in shape does not imply that allostery is not at play.
Tsai et al. (2008)
16 more