1woj

X-ray diffraction
1.8Å resolution

Crystal structure of human phosphodiesterase

Released:

Function and Biology Details

Reaction catalysed:
Nucleoside 2',3'-cyclic phosphate + H(2)O = nucleoside 2'-phosphate
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-140690 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2',3'-cyclic-nucleotide 3'-phosphodiesterase Chain: A
Molecule details ›
Chain: A
Length: 214 amino acids
Theoretical weight: 23.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P09543 (Residues: 186-399; Coverage: 51%)
Gene name: CNP
Sequence domains: 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNP or CNPase)
Structure domains: 2',3'-cyclic nucleotide 3'-phosphodiesterase superfamily

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P212121
Unit cell:
a: 44.386Å b: 55.348Å c: 78.757Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.192 0.225
Expression system: Escherichia coli BL21(DE3)