› 1wqw

X-ray diffraction
1.45Å resolution

Crystal Structure Of Biotin Protein Ligase From Pyrococcus Horikoshii Ot3 in complex with Biotinyl-5-AMP

Released:
DOI: 10.2210/pdb1wqw/pdb
PDB entry 1wqw coloured by chain, front view.
PDB entry 1wqw coloured by chain, side view.
PDB entry 1wqw coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Primary publication:
Crystal structures of biotin protein ligase from Pyrococcus horikoshii OT3 and its complexes: structural basis of biotin activation.
Bagautdinov B, Kuroishi C, Sugahara M, Kunishima N
J Mol Biol 353 322-33 (2005)
PMID: 16169557

Function and Biology Details

Reaction catalysed: 
ATP + biotin + [biotin carboxyl-carrier protein]-L-lysine = AMP + diphosphate + [biotin carboxyl-carrier protein]-N(6)-biotinyl-L-lysine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129627 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
BPL/LPL catalytic domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 235 amino acids
Theoretical weight: 26.1 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli
UniProt:
  • Canonical: O57883 (Residues: 1-235; Coverage: 100%)
Gene name: PH0147
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand BT5 2 x BT5
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P21
Unit cell:
a: 38.348Å b: 82.832Å c: 72.785Å
α: 90° β: 102.15° γ: 90°
R-values:
R R work R free
0.212 0.212 0.24
Expression system: Escherichia coli

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Citations

4 review citations

Vitamin and cofactor biosynthesis pathways in Plasmodium and other apicomplexan parasites.
Müller et al. (2007)
3 more

4 mentions without citation

Modulation of epigenetic targets for anticancer therapy: clinicopathological relevance, structural data and drug discovery perspectives.
Andreoli et al. (2013)
3 more