› 1wro

X-ray diffraction
2.35Å resolution

Metal Ion dependency of the antiterminator protein, HutP, for binding to the terminator region of hut mRNA- A structural basis

Released:
DOI: 10.2210/pdb1wro/pdb
PDB entry 1wro coloured by chain, front view.
PDB entry 1wro coloured by chain, side view.
PDB entry 1wro coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Characterization of the metal ion binding site in the anti-terminator protein, HutP, of Bacillus subtilis.
Kumarevel T, Mizuno H, Kumar PK
OpenAccess logo Nucleic Acids Res 33 5494-502 (2005)
PMID: 16192572

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145533 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hut operon positive regulatory protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 147 amino acids
Theoretical weight: 16.1 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P10943 (Residues: 2-148; Coverage: 99%)
Gene names: BSU39340, hutP
Sequence domains: HutP
Structure domains: Hut operon regulatory protein HutP

Ligands and Environments

2 bound ligands:
Ligand BA 6 x BA
Ligand HIS 3 x HIS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P21212
Unit cell:
a: 78.22Å b: 81.14Å c: 75.97Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.247 0.247 0.3
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Structural insights of HutP-mediated regulation of transcription of the hut operon in Bacillus subtilis.
Kumarevel T. (2007)
9 other articles

6 mentions without citation

Metal ion-dependent anti-termination of transcriptional regulation of ribonucleoprotein complexes.
Kumar et al. (2014)
5 more