› 1wxi

X-ray diffraction
1.7Å resolution

E.coli NAD Synthetase, AMP.PP

Released:
DOI: 10.2210/pdb1wxi/pdb
PDB entry 1wxi coloured by chain, front view.
PDB entry 1wxi coloured by chain, side view.
PDB entry 1wxi coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structures of Escherichia coli NAD synthetase with substrates and products reveal mechanistic rearrangements.
Jauch R, Humm A, Huber R, Wahl MC
J Biol Chem 280 15131-40 (2005)
PMID: 15699042

Function and Biology Details

Reaction catalysed: 
ATP + deamido-NAD(+) + NH(3) = AMP + diphosphate + NAD(+)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148454 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NH(3)-dependent NAD(+) synthetase Chain: A
Molecule details ›
Chain: A
Length: 275 amino acids
Theoretical weight: 30.67 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P18843 (Residues: 1-275; Coverage: 100%)
Gene names: JW1729, b1740, efg, nadE, ntrL
Sequence domains: NAD synthase
Structure domains: HUPs

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand AMP 2 x AMP
Ligand DPO 1 x DPO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2
Unit cell:
a: 92.444Å b: 67.922Å c: 48.394Å
α: 90° β: 104.24° γ: 90°
R-values:
R R work R free
0.176 0.176 0.205
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Nicotinamide adenine dinucleotide metabolism as an attractive target for drug discovery.
Khan et al. (2007)
1 more

2 mentions without citation

Streptococcus pyogenes quinolinate-salvage pathway-structural and functional studies of quinolinate phosphoribosyl transferase and NH3 -dependent NAD+ synthetase.
Booth et al. (2017)
1 more