Function and Biology

Improved Crystal Structure of Isopullulanase from Aspergillus niger ATCC 9642

Source organism: Aspergillus niger
Biochemical function: isopullulanase activity
Biological process: not assigned
Cellular component: extracellular region

EC 3.2.1.57: Isopullulanase

Reaction catalysed:
Hydrolysis of pullulan to isopanose (6-alpha-maltosylglucose)
Systematic name:
Pullulan 4-glucanohydrolase (isopanose-forming)

Sequence families

Pfam Protein families (Pfam)
PF03718
Domain description: Glycosyl hydrolase family 49
Occurring in:
  1. Isopullulanase
The deposited structure of PDB entry 1x0c contains 2 copies of Pfam domain PF03718 (Glycosyl hydrolase family 49) in Isopullulanase. Showing 1 copy in chain A.

PF18783
Domain description: Isopullulanase beta-solenoid repeat
Occurring in:
  1. Isopullulanase
The deposited structure of PDB entry 1x0c contains 2 copies of Pfam domain PF18783 (Isopullulanase beta-solenoid repeat) in Isopullulanase. Showing 1 copy in chain A.

PF18841
Domain description: Beta solenoid repeat from Dextranase
Occurring in:
  1. Isopullulanase
The deposited structure of PDB entry 1x0c contains 2 copies of Pfam domain PF18841 (Beta solenoid repeat from Dextranase) in Isopullulanase. Showing 1 copy in chain A.

PF17433
Domain description: Glycosyl hydrolase family 49 N-terminal Ig-like domain
Occurring in:
  1. Isopullulanase
The deposited structure of PDB entry 1x0c contains 2 copies of Pfam domain PF17433 (Glycosyl hydrolase family 49 N-terminal Ig-like domain) in Isopullulanase. Showing 1 copy in chain A.

InterPro InterPro annotations
IPR035953
Domain description: Dextranase, N-terminal
Occurring in:
  1. Isopullulanase
IPR005192
Domain description: Glycoside hydrolase, family 49, C-terminal
Occurring in:
  1. Isopullulanase
IPR041274
Domain description: Isopullulanase beta-solenoid repeat
Occurring in:
  1. Isopullulanase
IPR041402
Domain description: Dextranase, beta solenoid repeat
Occurring in:
  1. Isopullulanase
IPR011050
Domain description: Pectin lyase fold/virulence factor
Occurring in:
  1. Isopullulanase
IPR012334
Domain description: Pectin lyase fold
Occurring in:
  1. Isopullulanase
IPR023226
Domain description: Glycoside hydrolase, family 49, N-terminal domain
Occurring in:
  1. Isopullulanase

Structure domains

CATH CATH domains
2.160.20.10
Class: Mainly Beta
Architecture: 3 Solenoid
Topology: Pectate Lyase C-like
Homology: Single-stranded right-handed beta-helix, Pectin lyase-like
Occurring in:
  1. Isopullulanase
The deposited structure of PDB entry 1x0c contains 2 copies of CATH domain 2.160.20.10 (Pectate Lyase C-like) in Isopullulanase. Showing 1 copy in chain A.
2.60.350.10
Class: Mainly Beta
Architecture: Sandwich
Topology: Dex49a from penicillium minioluteum complex, domain 1
Homology: Dextranase, N-terminal
Occurring in:
  1. Isopullulanase
The deposited structure of PDB entry 1x0c contains 2 copies of CATH domain 2.60.350.10 (Dex49a from penicillium minioluteum complex, domain 1) in Isopullulanase. Showing 1 copy in chain A.