› 1x9e

X-ray diffraction
2.4Å resolution

Crystal structure of HMG-CoA synthase from Enterococcus faecalis

Released:
DOI: 10.2210/pdb1x9e/pdb
PDB entry 1x9e coloured by chain, front view.
PDB entry 1x9e coloured by chain, side view.
PDB entry 1x9e coloured by chain, top view.
Source organism: Enterococcus faecalis
Primary publication:
X-ray crystal structures of HMG-CoA synthase from Enterococcus faecalis and a complex with its second substrate/inhibitor acetoacetyl-CoA.
Steussy CN, Vartia AA, Burgner JW, Sutherlin A, Rodwell VW, Stauffacher CV
Biochemistry 44 14256-67 (2005)
PMID: 16245942

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + H(2)O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190291 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hydroxymethylglutaryl-CoA synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 383 amino acids
Theoretical weight: 42.19 KDa
Source organism: Enterococcus faecalis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9FD71 (Residues: 1-383; Coverage: 100%)
Gene name: mvaS
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-ID-B
Spacegroup: I222
Unit cell:
a: 105.368Å b: 109.82Å c: 141.54Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.225 0.254
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Enzymes of the mevalonate pathway of isoprenoid biosynthesis.
Miziorko HM. (2011)
2 more

2 mentions without citation

Biochemical and structural basis for inhibition of Enterococcus faecalis hydroxymethylglutaryl-CoA synthase, mvaS, by hymeglusin.
Skaff et al. (2012)
1 more