› 1xg6

X-ray diffraction
2.15Å resolution

The crystal structure of the P1 mutant (Leu to Arg)of a Winged bean chymotrypsin inhibitor(Kunitz)solved at 2.15A resolution

Released:
DOI: 10.2210/pdb1xg6/pdb
PDB entry 1xg6 coloured by chain, front view.
PDB entry 1xg6 coloured by chain, side view.
PDB entry 1xg6 coloured by chain, top view.
Source organism: Psophocarpus tetragonolobus
Primary publication:
Single mutation at P1 of a chymotrypsin inhibitor changes it to a trypsin inhibitor: X-ray structural (2.15 A) and biochemical basis.
Khamrui S, Dasgupta J, Dattagupta JK, Sen U
Biochim Biophys Acta 1752 65-72 (2005)
PMID: 16081330

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145463 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chymotrypsin inhibitor 3 Chain: A
Molecule details ›
Chain: A
Length: 187 amino acids
Theoretical weight: 20.72 KDa
Source organism: Psophocarpus tetragonolobus
Expression system: Escherichia coli
UniProt:
  • Canonical: P10822 (Residues: 25-207; Coverage: 100%)
Sequence domains: Trypsin and protease inhibitor
Structure domains: Trefoil (Acidic Fibroblast Growth Factor, subunit A)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P65
Unit cell:
a: 99.57Å b: 99.57Å c: 37.261Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.199 0.199 0.236
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Structural and functional diversities in lepidopteran serine proteases.
Srinivasan et al. (2006)
2 more

1 mention without citation

Identification of a novel set of scaffolding residues that are instrumental for the inhibitory property of Kunitz (STI) inhibitors.
Khamrui et al. (2010)