› 1xgp

X-ray diffraction
2.1Å resolution

Structure for antibody HyHEL-63 Y33A mutant complexed with hen egg lysozyme

Released:
DOI: 10.2210/pdb1xgp/pdb
PDB entry 1xgp coloured by chain, front view.
PDB entry 1xgp coloured by chain, side view.
PDB entry 1xgp coloured by chain, top view.
Source organisms:
Primary publication:
Magnitude of the hydrophobic effect at central versus peripheral sites in protein-protein interfaces.
Li Y, Huang Y, Swaminathan CP, Smith-Gill SJ, Mariuzza RA
Structure 13 297-307 (2005)
PMID: 15698573

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208250 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
antibody kappa light chain Chain: A
Molecule details ›
Chain: A
Length: 215 amino acids
Theoretical weight: 23.72 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
antibody kappa heavy chain Chain: B
Molecule details ›
Chain: B
Length: 210 amino acids
Theoretical weight: 22.46 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Lysozyme C Chain: C
Molecule details ›
Chain: C
Length: 129 amino acids
Theoretical weight: 14.33 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00698 (Residues: 19-147; Coverage: 100%)
Gene name: LYZ
Sequence domains: C-type lysozyme/alpha-lactalbumin family
Structure domains: Lysozyme

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P42212
Unit cell:
a: 90.316Å b: 90.316Å c: 150.592Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.215 0.271
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Protein-protein interaction and quaternary structure.
Janin et al. (2008)
2 more

1 mention without citation

The molecular basis for modulation of human Vγ9Vδ2 T cell responses by CD277/butyrophilin-3 (BTN3A)-specific antibodies.
Palakodeti et al. (2012)