› 1xkf

X-ray diffraction
1.9Å resolution

Crystal structure of Hypoxic Response Protein I (HRPI) with two coordinated zinc ions

Released:
DOI: 10.2210/pdb1xkf/pdb
PDB entry 1xkf coloured by chain, front view.
PDB entry 1xkf coloured by chain, side view.
PDB entry 1xkf coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
The structure and unusual protein chemistry of hypoxic response protein 1, a latency antigen and highly expressed member of the DosR regulon in Mycobacterium tuberculosis.
Sharpe ML, Gao C, Kendall SL, Baker EN, Lott JS
J Mol Biol 383 822-36 (2008)
PMID: 18640126

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161677 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hypoxic response protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 133 amino acids
Theoretical weight: 14.5 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P9WJA3 (Residues: 1-127; Coverage: 89%)
Gene names: Rv2626c, hrp1
Sequence domains: CBS domain
Structure domains: CBS-domain

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P212121
Unit cell:
a: 40.65Å b: 50.94Å c: 129.19Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.206 0.235
Expression system: Escherichia coli BL21

Quick links

Citations

3 review citations

CBS domains: Ligand binding sites and conformational variability.
Ereño-Orbea et al. (2013)
2 more

1 mention without citation

Biochemical and structural characterization of a novel family of cystathionine beta-synthase domain proteins fused to a Zn ribbon-like domain.
Proudfoot et al. (2008)