› 1xld

X-ray diffraction
2.5Å resolution

MECHANISM FOR ALDOSE-KETOSE INTERCONVERSION BY D-XYLOSE ISOMERASE INVOLVING RING OPENING FOLLOWED BY A 1,2-HYDRIDE SHIFT

Released:
DOI: 10.2210/pdb1xld/pdb
PDB entry 1xld coloured by chain, front view.
PDB entry 1xld coloured by chain, side view.
PDB entry 1xld coloured by chain, top view.
Source organism: Arthrobacter sp. NRRL B3728
Primary publication:
Mechanism for aldose-ketose interconversion by D-xylose isomerase involving ring opening followed by a 1,2-hydride shift.
Collyer CA, Henrick K, Blow DM
J Mol Biol 212 211-35 (1990)
PMID: 2319597

Function and Biology Details

Reaction catalysed: 
Alpha-D-xylopyranose = alpha-D-xylufuranose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146092 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Xylose isomerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 394 amino acids
Theoretical weight: 43.21 KDa
Source organism: Arthrobacter sp. NRRL B3728
Expression system: Not provided
UniProt:
  • Canonical: P12070 (Residues: 2-395; Coverage: 100%)
Gene name: xylA
Sequence domains: Xylose isomerase-like TIM barrel
Structure domains: Divalent-metal-dependent TIM barrel enzymes

Ligands and Environments

2 bound ligands:
Ligand XYL 2 x XYL
Ligand MN 4 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P3121
Unit cell:
a: 105.6Å b: 105.6Å c: 153.5Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.148 not available not available
Expression system: Not provided

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Citations

3 review citations

Molecular and industrial aspects of glucose isomerase.
Bhosale et al. (1996)
2 more