› 1xlp

X-ray diffraction
2Å resolution

Structure of oxidized C73S putidaredoxin from Pseudomonas putida

Released:
DOI: 10.2210/pdb1xlp/pdb
PDB entry 1xlp coloured by chain, front view.
PDB entry 1xlp coloured by chain, side view.
PDB entry 1xlp coloured by chain, top view.
Source organism: Pseudomonas putida
Primary publication:
Redox-dependent structural reorganization in putidaredoxin, a vertebrate-type [2Fe-2S] ferredoxin from Pseudomonas putida.
Sevrioukova IF
J Mol Biol 347 607-21 (2005)
PMID: 15755454

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-132340 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putidaredoxin Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 106 amino acids
Theoretical weight: 11.41 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00259 (Residues: 2-107; Coverage: 99%)
Gene name: camB
Sequence domains: 2Fe-2S iron-sulfur cluster binding domain
Structure domains: Ubiquitin-like (UB roll)

Ligands and Environments

1 bound ligand:
Ligand FES 3 x FES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P41212
Unit cell:
a: 66.22Å b: 66.22Å c: 151.751Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.217 0.221
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Structural biology of redox partner interactions in P450cam monooxygenase: a fresh look at an old system.
Sevrioukova et al. (2011)
2 more

3 mentions without citation

Putidaredoxin-to-cytochrome P450cam electron transfer: differences between the two reductive steps required for catalysis.
Kuznetsov et al. (2006)
2 more