› 1xwr

X-ray diffraction
2.56Å resolution

Crystal structure of the coliphage lambda transcription activator protein CII

Released:
DOI: 10.2210/pdb1xwr/pdb
PDB entry 1xwr coloured by chain, front view.
PDB entry 1xwr coloured by chain, side view.
PDB entry 1xwr coloured by chain, top view.
Source organism: Escherichia virus Lambda
Primary publication:
Structure of lambda CII: implications for recognition of direct-repeat DNA by an unusual tetrameric organization.
Datta AB, Panjikar S, Weiss MS, Chakrabarti P, Parrack P
Proc Natl Acad Sci U S A 102 11242-7 (2005)
PMID: 16061804

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136480 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcriptional activator II Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 97 amino acids
Theoretical weight: 11.07 KDa
Source organism: Escherichia virus Lambda
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03042 (Residues: 1-97; Coverage: 100%)
Gene names: cII, lambdap59
Sequence domains: Bacteriophage CII protein
Structure domains: lambda repressor-like DNA-binding domains

Ligands and Environments

1 bound ligand:
Ligand IPA 3 x IPA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A, EMBL/DESY, HAMBURG BEAMLINE X13, SPRING-8 BEAMLINE BL40B2
Spacegroup: C2221
Unit cell:
a: 63.907Å b: 106.794Å c: 119.784Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.315 0.25 0.286
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Hybrid Vigor: Importance of Hybrid λ Phages in Early Insights in Molecular Biology.
Feiss et al. (2022)
9 other articles

3 mentions without citation

The protein interaction map of bacteriophage lambda.
Rajagopala et al. (2011)
2 more