› 1y66

X-ray diffraction
1.65Å resolution

Dioxane contributes to the altered conformation and oligomerization state of a designed engrailed homeodomain variant

Released:
DOI: 10.2210/pdb1y66/pdb
Model geometry
Fit model/data
PDB entry 1y66 coloured by chain, front view.
PDB entry 1y66 coloured by chain, side view.
PDB entry 1y66 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Dioxane contributes to the altered conformation and oligomerization state of a designed engrailed homeodomain variant.
Hom GK, Lassila JK, Thomas LM, Mayo SL
Protein Sci 14 1115-9 (2005)
PMID: 15741348

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-162559 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
engrailed homeodomain Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 52 amino acids
Theoretical weight: 6.59 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
Structure domains: Helix Hairpins

Ligands and Environments

3 bound ligands:
Ligand CD 2 x CD
Ligand DIO 10 x DIO
Ligand ACY 1 x ACY
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: RIGAKU RU300
Spacegroup: P212121
Unit cell:
a: 50.712Å b: 52.646Å c: 82.182Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.187 0.228
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Full-sequence computational design and solution structure of a thermostable protein variant.
Shah et al. (2007)
1 more

3 mentions without citation

Dioxane contributes to the altered conformation and oligomerization state of a designed engrailed homeodomain variant.
Hom et al. (2005)
2 more