› 1y7a

X-ray diffraction
1.77Å resolution

Structure of D153H/K328W E. coli alkaline phosphatase in presence of cobalt at 1.77 A resolution

Released:
DOI: 10.2210/pdb1y7a/pdb
PDB entry 1y7a coloured by chain, front view.
PDB entry 1y7a coloured by chain, side view.
PDB entry 1y7a coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Metal specificity is correlated with two crucial active site residues in Escherichia coli alkaline phosphatase.
Wang J, Stieglitz KA, Kantrowitz ER
Biochemistry 44 8378-86 (2005)
PMID: 15938627

Function and Biology Details

Reaction catalysed: 
A phosphate monoester + H(2)O = an alcohol + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132772 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alkaline phosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 449 amino acids
Theoretical weight: 47.17 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P00634 (Residues: 23-471; Coverage: 100%)
Gene names: JW0374, b0383, phoA
Sequence domains: Alkaline phosphatase
Structure domains: Alkaline Phosphatase, subunit A

Ligands and Environments

3 bound ligands:
Ligand CO 6 x CO
Ligand PO4 2 x PO4
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: I222
Unit cell:
a: 76.208Å b: 164.564Å c: 192.636Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.205 0.238
Expression system: Escherichia coli

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Citations

1 review citation

Prosthetic Metals: Release, Metabolism and Toxicity.
Zhong et al. (2024)
21 other articles