› 1ytf

X-ray diffraction
2.5Å resolution

YEAST TFIIA/TBP/DNA COMPLEX

Released:
DOI: 10.2210/pdb1ytf/pdb
Model geometry
Fit model/data
Data not deposited
PDB entry 1ytf coloured by chain, front view.
PDB entry 1ytf coloured by chain, side view.
PDB entry 1ytf coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Crystal structure of a yeast TFIIA/TBP/DNA complex.
Tan S, Hunziker Y, Sargent DF, Richmond TJ
Nature 381 127-51 (1996)
PMID: 8610010

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114873 (preferred)
Entry contents:
4 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (6 distinct):
TATA-box-binding protein Chain: A
Molecule details ›
Chain: A
Length: 180 amino acids
Theoretical weight: 20.12 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P13393 (Residues: 61-240; Coverage: 75%)
Gene names: BTF1, SPT15, TBP1, YER148W
Sequence domains: Transcription factor TFIID (or TATA-binding protein, TBP)
Structure domains: TATA-Binding Protein
Transcription initiation factor IIA large subunit Chain: B
Molecule details ›
Chain: B
Length: 53 amino acids
Theoretical weight: 6.14 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P32773 (Residues: 2-54; Coverage: 19%)
Gene names: TOA1, YOR194C
Structure domains: Helix Hairpins
Transcription initiation factor IIA large subunit Chain: C
Transcription initiation factor IIA subunit 2 Chain: D
DNA (5'-D(*TP*GP*TP*AP*TP*GP*TP*AP*TP*AP*TP*AP*AP*AP*AP*C)-3') Chain: E
DNA (5'-D(*GP*TP*TP*TP*TP*AP*TP*AP*TP*AP*CP*AP*TP*AP*CP*A)-3') Chain: F

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P212121
Unit cell:
a: 59.15Å b: 93.05Å c: 117.83Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.235 0.297
Expression system: Escherichia coli

Quick links

Citations

37 review citations

The general transcription machinery and general cofactors.
Thomas et al. (2006)
36 more

13 mentions without citation

ProBiS algorithm for detection of structurally similar protein binding sites by local structural alignment.
Konc et al. (2010)
12 more