› 1yu9

X-ray diffraction
2.07Å resolution

GppNHp-Bound Rab4A

Released:
DOI: 10.2210/pdb1yu9/pdb
PDB entry 1yu9 coloured by chain, front view.
PDB entry 1yu9 coloured by chain, side view.
PDB entry 1yu9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of family-wide Rab GTPase recognition by rabenosyn-5.
Eathiraj S, Pan X, Ritacco C, Lambright DG
Nature 436 415-9 (2005)
PMID: 16034420

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-539464 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ras-related protein Rab-4A Chain: A
Molecule details ›
Chain: A
Length: 175 amino acids
Theoretical weight: 19.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P20338 (Residues: 7-177; Coverage: 78%)
Gene names: RAB4, RAB4A
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand MG 1 x MG
Ligand SO4 2 x SO4
Ligand GNP 1 x GNP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P43212
Unit cell:
a: 68.808Å b: 68.808Å c: 90.92Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.156 0.195
Expression system: Escherichia coli

Quick links

Citations

29 review citations

Rab GTPases as coordinators of vesicle traffic.
Stenmark H. (2009)
28 more

6 mentions without citation

Structural mechanisms for regulation of membrane traffic by rab GTPases.
Lee et al. (2009)
5 more