› 1yun

X-ray diffraction
2Å resolution

Crystal Structure of Nicotinic Acid Mononucleotide Adenylyltransferase from Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb1yun/pdb
PDB entry 1yun coloured by chain, front view.
PDB entry 1yun coloured by chain, side view.
PDB entry 1yun coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Crystal structure of nicotinic acid mononucleotide adenylyltransferase from Pseudomonas aeruginosa in its Apo and substrate-complexed forms reveals a fully open conformation.
Yoon HJ, Kim HL, Mikami B, Suh SW
J Mol Biol 351 258-65 (2005)
PMID: 16009375

Function and Biology Details

Reaction catalysed: 
ATP + beta-nicotinate-D-ribonucleotide = diphosphate + deamido-NAD(+)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-574858 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable nicotinate-nucleotide adenylyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 242 amino acids
Theoretical weight: 27.07 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HX21 (Residues: 1-214; Coverage: 100%)
Gene names: PA4006, nadD
Sequence domains: Cytidylyltransferase-like
Structure domains: HUPs

Ligands and Environments

2 bound ligands:
Ligand ATP 2 x ATP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-18B
Spacegroup: P2221
Unit cell:
a: 65.189Å b: 65.199Å c: 110.121Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.172 0.243
Expression system: Escherichia coli

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Citations

3 review citations

Nicotinamide/nicotinic acid mononucleotide adenylyltransferase, new insights into an ancient enzyme.
Zhai et al. (2009)
2 more

3 mentions without citation

Mycobacterial nicotinate mononucleotide adenylyltransferase: structure, mechanism, and implications for drug discovery.
Rodionova et al. (2015)
2 more