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Saccharomyces cerevisiae Ste50 binds the MAPKKK Ste11 through a head-to-tail SAM domain interaction.

Kwan JJ, Warner N, Maini J, Chan Tung KW, Zakaria H, Pawson T, Donaldson LW
J Mol Biol 356 142-54 (2006)
Cited: 25 times
EuropePMC logo PMID: 16337230

Abstract

In Saccharomyces cerevisiae, signal transduction through pathways governing mating, osmoregulation, and nitrogen starvation depends upon a direct interaction between the sterile alpha motif (SAM) domains of the Ste11 mitogen-activated protein kinase kinase kinase (MAPKKK) and its regulator Ste50. Previously, we solved the NMR structure of the SAM domain from Ste11 and identified two mutants that diminished binding to the Ste50 SAM domain. Building upon the Ste11 study, we present the NMR structure of the monomeric Ste50 SAM domain and a series of mutants bearing substitutions at surface-exposed hydrophobic amino acid residues. The mid-loop (ML) region of Ste11-SAM, defined by helices H3 and H4 and the end-helix (EH) region of Ste50-SAM, defined by helix H5, were sensitive to substitution, indicating that these two surfaces contribute to the high-affinity interaction. The combination of two mutants, Ste11-SAM-L72R and Ste50-SAM-L69R, formed a high-affinity heterodimer unencumbered by competing homotypic interactions that had prevented earlier NMR studies of the wild-type complex. Yeast bearing mutations that prevented the heterotypic Ste11-Ste50 association in vitro presented signaling defects in the mating and high-osmolarity growth pathways.

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Articles - 1z1v mentioned but not cited (2)

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  1. Function and regulation in MAPK signaling pathways: lessons learned from the yeast Saccharomyces cerevisiae. Chen RE, Thorner J. Biochim Biophys Acta 1773 1311-1340 (2007)
  2. Response to hyperosmotic stress. Saito H, Posas F. Genetics 192 289-318 (2012)
  3. Heterotrimeric G Protein-coupled Receptor Signaling in Yeast Mating Pheromone Response. Alvaro CG, Thorner J. J Biol Chem 291 7788-7795 (2016)

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  2. Multiple upstream signals converge on the adaptor protein Mst50 in Magnaporthe grisea. Park G, Xue C, Zhao X, Kim Y, Orbach M, Xu JR. Plant Cell 18 2822-2835 (2006)
  3. A role for zinc in postsynaptic density asSAMbly and plasticity? Gundelfinger ED, Boeckers TM, Baron MK, Bowie JU. Trends Biochem Sci 31 366-373 (2006)
  4. Structural evolution of C-terminal domains in the p53 family. Ou HD, Löhr F, Vogel V, Mäntele W, Dötsch V. EMBO J 26 3463-3473 (2007)
  5. The transmembrane protein FgSho1 regulates fungal development and pathogenicity via the MAPK module Ste50-Ste11-Ste7 in Fusarium graminearum. Gu Q, Chen Y, Liu Y, Zhang C, Ma Z. New Phytol 206 315-328 (2015)
  6. Survey of the year 2006 commercial optical biosensor literature. Rich RL, Myszka DG. J Mol Recognit 20 300-366 (2007)
  7. The PI3K effector Arap3 interacts with the PI(3,4,5)P3 phosphatase SHIP2 in a SAM domain-dependent manner. Raaijmakers JH, Deneubourg L, Rehmann H, de Koning J, Zhang Z, Krugmann S, Erneux C, Bos JL. Cell Signal 19 1249-1257 (2007)
  8. MST50 is involved in multiple MAP kinase signaling pathways in Magnaporthe oryzae. Li G, Zhang X, Tian H, Choi YE, Tao WA, Xu JR. Environ Microbiol 19 1959-1974 (2017)
  9. SAM domain-based protein oligomerization observed by live-cell fluorescence fluctuation spectroscopy. Slaughter BD, Huff JM, Wiegraebe W, Schwartz JW, Li R. PLoS One 3 e1931 (2008)
  10. NMR structural studies of the Ste11 SAM domain in the dodecyl phosphocholine micelle. Bhunia A, Domadia PN, Mohanram H, Bhattacharjya S. Proteins 74 328-343 (2009)
  11. The Saccharomyces cerevisiae flavodoxin-like proteins Ycp4 and Rfs1 play a role in stress response and in the regulation of genes related to metabolism. Cardona F, Orozco H, Friant S, Aranda A, del Olmo Ml. Arch Microbiol 193 515-525 (2011)
  12. The robustness of a signaling complex to domain rearrangements facilitates network evolution. Sato PM, Yoganathan K, Jung JH, Peisajovich SG. PLoS Biol 12 e1002012 (2014)
  13. A new mode of SAM domain mediated oligomerization observed in the CASKIN2 neuronal scaffolding protein. Smirnova E, Kwan JJ, Siu R, Gao X, Zoidl G, Demeler B, Saridakis V, Donaldson LW. Cell Commun Signal 14 17 (2016)
  14. CASCADE_SCAN: mining signal transduction network from high-throughput data based on steepest descent method. Wang K, Hu F, Xu K, Cheng H, Jiang M, Feng R, Li J, Wen T. BMC Bioinformatics 12 164 (2011)
  15. Role of phosphatidylinositol phosphate signaling in the regulation of the filamentous-growth mitogen-activated protein kinase pathway. Adhikari H, Cullen PJ. Eukaryot Cell 14 427-440 (2015)
  16. Silencing PsKPP4, a MAP kinase kinase kinase gene, reduces pathogenicity of the stripe rust fungus. Zhu X, Guo J, He F, Zhang Y, Tan C, Yang Q, Huang C, Kang Z, Guo J. Mol Plant Pathol 19 2590-2602 (2018)
  17. Cdc42-Specific GTPase-Activating Protein Rga1 Squelches Crosstalk between the High-Osmolarity Glycerol (HOG) and Mating Pheromone Response MAPK Pathways. Patterson JC, Goupil LS, Thorner J. Biomolecules 11 1530 (2021)
  18. The Adaptor Protein UvSte50 Governs Fungal Pathogenicity of Ustilaginoidea virens via the MAPK Signaling Pathway. Cao H, Gong H, Song T, Yu M, Pan X, Yu J, Qi Z, Du Y, Liu Y. J Fungi (Basel) 8 954 (2022)
  19. Characterization of the near native conformational states of the SAM domain of Ste11 protein by NMR spectroscopy. Gupta S, Bhattacharjya S. Proteins 82 2957-2969 (2014)


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