Function and Biology Details
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
- Ubiquitin-conjugating enzyme E2
- Ran-GTPase activating protein 1, C-terminal domain superfamily
- Ubiquitin-like domain
- Ubiquitin-conjugating enzyme/RWD-like
- Nup358/RanBP2 E3 ligase domain
- Ran-GTPase activating protein 1, C-terminal
- Rad60/SUMO-like domain
- Small ubiquitin-related modifier 1, Ubl domain
2 more domains
Structure analysis Details
Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155409 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
SUMO-conjugating enzyme UBC9
Molecule details ›
Chain: A
Length: 158 amino acids
Theoretical weight: 18.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Ubiquitin-conjugating enzyme
Structure domains: Ubiquitin Conjugating Enzyme
Length: 158 amino acids
Theoretical weight: 18.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
P63279 (Residues: 1-158; Coverage: 100%)
Sequence domains: Ubiquitin-conjugating enzyme
Structure domains: Ubiquitin Conjugating Enzyme
Small ubiquitin-related modifier 1
Molecule details ›
Chain: B
Length: 82 amino acids
Theoretical weight: 9.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Ubiquitin-2 like Rad60 SUMO-like
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
Length: 82 amino acids
Theoretical weight: 9.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: P63165 (Residues: 18-97; Coverage: 79%)
Sequence domains: Ubiquitin-2 like Rad60 SUMO-like
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
Ran GTPase-activating protein 1
Molecule details ›
Chain: C
Length: 172 amino acids
Theoretical weight: 18.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: RanGAP1 C-terminal domain
Structure domains: Ran-GTPase activating protein 1, C-terminal domain
Length: 172 amino acids
Theoretical weight: 18.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: P46060 (Residues: 418-587; Coverage: 29%)
Sequence domains: RanGAP1 C-terminal domain
Structure domains: Ran-GTPase activating protein 1, C-terminal domain
E3 SUMO-protein ligase RanBP2
Molecule details ›
Chain: D
Length: 83 amino acids
Theoretical weight: 9.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Nup358/RanBP2 E3 ligase domain
Length: 83 amino acids
Theoretical weight: 9.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: P49792 (Residues: 2631-2711; Coverage: 3%)
Sequence domains: Nup358/RanBP2 E3 ligase domain
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
APS BEAMLINE 31-ID
Spacegroup:
P3221
Expression system: Escherichia coli BL21(DE3)
