› 1zat

X-ray diffraction
2.4Å resolution

Crystal Structure of an Enterococcus faecium peptidoglycan binding protein at 2.4 A resolution

Released:
DOI: 10.2210/pdb1zat/pdb
PDB entry 1zat coloured by chain, front view.
PDB entry 1zat coloured by chain, side view.
PDB entry 1zat coloured by chain, top view.
Source organism: Enterococcus faecium
Primary publication:
Crystal structure of a novel beta-lactam-insensitive peptidoglycan transpeptidase.
Biarrotte-Sorin S, Hugonnet JE, Delfosse V, Mainardi JL, Gutmann L, Arthur M, Mayer C
J Mol Biol 359 533-8 (2006)
PMID: 16647082

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174881 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L,D-TPase catalytic domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 250 amino acids
Theoretical weight: 27.9 KDa
Source organism: Enterococcus faecium
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q3Y185 (Residues: 217-466; Coverage: 54%)
Gene name: HMPREF0351_12555
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P3121
Unit cell:
a: 115.976Å b: 115.976Å c: 68.275Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.34 0.22 0.257
Expression system: Escherichia coli BL21(DE3)

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Citations

15 review citations

The Enterococcus: a Model of Adaptability to Its Environment.
García-Solache et al. (2019)
14 more

8 mentions without citation

Polymorphic toxin systems: Comprehensive characterization of trafficking modes, processing, mechanisms of action, immunity and ecology using comparative genomics.
Zhang et al. (2012)
7 more