› 1zes

X-ray diffraction
1.9Å resolution

BeF3- activated PhoB receiver domain

Released:
DOI: 10.2210/pdb1zes/pdb
PDB entry 1zes coloured by chain, front view.
PDB entry 1zes coloured by chain, side view.
PDB entry 1zes coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Mechanism of activation for transcription factor PhoB suggested by different modes of dimerization in the inactive and active states.
Bachhawat P, Swapna GV, Montelione GT, Stock AM
Structure 13 1353-63 (2005)
PMID: 16154092

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-533223 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphate regulon transcriptional regulatory protein PhoB Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 125 amino acids
Theoretical weight: 14.34 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0AFJ5 (Residues: 1-125; Coverage: 55%)
Gene names: JW0389, b0399, phoB
Sequence domains: Response regulator receiver domain
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand MG 3 x MG
Ligand BEF 3 x BEF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P43212
Unit cell:
a: 77.869Å b: 77.869Å c: 109.071Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.189 0.24
Expression system: Escherichia coli BL21

Quick links

Citations

9 review citations

Global regulation by the seven-component Pi signaling system.
Hsieh et al. (2010)
8 more

24 mentions without citation

Molecular Mechanisms of Two-Component Signal Transduction.
Zschiedrich et al. (2016)
23 more