› 1zet

X-ray diffraction
2.3Å resolution

X-ray data do not support hoogsteen base-pairing during replication by human polymerase iota

Released:
DOI: 10.2210/pdb1zet/pdb
PDB entry 1zet coloured by chain, front view.
PDB entry 1zet coloured by chain, side view.
PDB entry 1zet coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
DNA polymerases: Hoogsteen base-pairing in DNA replication?
Wang J
Nature 437 E6-7; discussion E7 (2005)
PMID: 16163299

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-506690 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase iota Chain: A
Molecule details ›
Chain: A
Length: 388 amino acids
Theoretical weight: 43.88 KDa
Source organism: Homo sapiens
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q9UNA4 (Residues: 52-439; Coverage: 52%)
Gene names: POLI, RAD30B
Sequence domains:
Structure domains:
5'-D(P*AP*GP*GP*GP*(BRU)P*CP*CP*(BRU)P*(BRU)P*CP*CP*CP*CP*C)-3' Chain: T
Molecule details ›
Chain: T
Length: 14 nucleotides
Theoretical weight: 4.39 KDa
Source organism: Homo sapiens
Expression system: Not provided
5'-D(*GP*GP*GP*GP*GP*AP*AP*GP*GP*AP*CP*CP*(DOC))-3' Chain: P
Molecule details ›
Chain: P
Length: 13 nucleotides
Theoretical weight: 4.05 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand TTP 1 x TTP
2 modified residues:
Ligand BRU 3 x BRU
Ligand DOC 1 x DOC

Experiments and Validation Details

Entry percentile scores
Spacegroup: P6522
Unit cell:
a: 98.831Å b: 98.831Å c: 202.755Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.261 0.261 0.288
Expression systems:
  • Saccharomyces cerevisiae
  • Not provided

Quick links

Citations

5 review citations

DNA polymerases and human diseases.
Sweasy et al. (2006)
4 more

3 mentions without citation

Detect, correct, retract: How to manage incorrect structural models.
Wlodawer et al. (2018)
2 more