› 1zgx

X-ray diffraction
1.13Å resolution

Crystal structure of ribonuclease mutant

Released:
DOI: 10.2210/pdb1zgx/pdb
Model geometry
Fit model/data
PDB entry 1zgx coloured by chain, front view.
PDB entry 1zgx coloured by chain, side view.
PDB entry 1zgx coloured by chain, top view.
Source organism: Kitasatospora aureofaciens
Entry authors: Urbanikova L, Sevcik J

Function and Biology Details

Reaction catalysed: 
(1a) (RNA) containing guanosine = (RNA fragment)-3'-guanosine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA fragment)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138778 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Guanyl-specific ribonuclease Sa Chain: A
Molecule details ›
Chain: A
Length: 63 amino acids
Theoretical weight: 6.81 KDa
Source organism: Kitasatospora aureofaciens
Expression system: Escherichia coli
UniProt:
  • Canonical: P05798 (Residues: 1-63; Coverage: 66%)
Gene name: rnaSA
Sequence domains: ribonuclease
Guanyl-specific ribonuclease Sa Chain: B
Molecule details ›
Chain: B
Length: 33 amino acids
Theoretical weight: 3.79 KDa
Source organism: Kitasatospora aureofaciens
Expression system: Escherichia coli
UniProt:
  • Canonical: P05798 (Residues: 64-96; Coverage: 34%)
Gene name: rnaSA
Sequence domains: ribonuclease

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X31
Spacegroup: P212121
Unit cell:
a: 34.582Å b: 41.513Å c: 58.403Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.129 0.128 0.164
Expression system: Escherichia coli

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