› 1zh6

X-ray diffraction
2.5Å resolution

Crystal Structure of p-acetylphenylalanine-tRNA synthetase in complex with p-acetylphenylalanine

Released:
DOI: 10.2210/pdb1zh6/pdb
PDB entry 1zh6 coloured by chain, front view.
PDB entry 1zh6 coloured by chain, side view.
PDB entry 1zh6 coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii
Primary publication:
Structural characterization of a p-acetylphenylalanyl aminoacyl-tRNA synthetase.
Turner JM, Graziano J, Spraggon G, Schultz PG
J Am Chem Soc 127 14976-7 (2005)
PMID: 16248607

Function and Biology Details

Reaction catalysed: 
ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-176481 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine--tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 314 amino acids
Theoretical weight: 36.1 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q57834 (Residues: 1-306; Coverage: 100%)
Gene names: MJ0389, tyrS
Sequence domains: tRNA synthetases class I (W and Y)
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand 4AF 1 x 4AF
Ligand BME 2 x BME
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: P43212
Unit cell:
a: 103.362Å b: 103.362Å c: 70.837Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.209 0.278
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

Adding new chemistries to the genetic code.
Liu et al. (2010)
2 more

6 mentions without citation

An evolved aminoacyl-tRNA synthetase with atypical polysubstrate specificity.
Young et al. (2011)
5 more