› 2a4d

X-ray diffraction
1.69Å resolution

Structure of the human ubiquitin-conjugating enzyme E2 variant 1 (UEV-1)

Released:
DOI: 10.2210/pdb2a4d/pdb
PDB entry 2a4d coloured by chain, front view.
PDB entry 2a4d coloured by chain, side view.
PDB entry 2a4d coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A human ubiquitin conjugating enzyme (E2)-HECT E3 ligase structure-function screen.
Sheng Y, Hong JH, Doherty R, Srikumar T, Shloush J, Avvakumov GV, Walker JR, Xue S, Neculai D, Wan JW, Kim SK, Arrowsmith CH, Raught B, Dhe-Paganon S
Mol Cell Proteomics 11 329-41 (2012)
PMID: 22496338

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171663 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin-conjugating enzyme E2 variant 1 Chain: A
Molecule details ›
Chain: A
Length: 160 amino acids
Theoretical weight: 18.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q13404 (Residues: 7-147; Coverage: 96%)
  • Best match: Q13404-7 (Residues: 31-170)
Gene names: CROC1, P/OKcl.19, UBE2V, UBE2V1, UEV1
Sequence domains: Ubiquitin-conjugating enzyme
Structure domains: Ubiquitin Conjugating Enzyme

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E
Spacegroup: C2221
Unit cell:
a: 43.058Å b: 53.91Å c: 108.034Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.183 0.24
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

E2 enzymes: more than just middle men.
Stewart et al. (2016)
10 more

5 mentions without citation

Structural basis of generic versus specific E2-RING E3 interactions in protein ubiquitination.
Gundogdu et al. (2019)
4 more