› 2a6v

X-ray diffraction
1.52Å resolution

Crystal structure of Emp46p carbohydrate recognition domain (CRD), potassium-bound form

Released:
DOI: 10.2210/pdb2a6v/pdb
PDB entry 2a6v coloured by chain, front view.
PDB entry 2a6v coloured by chain, side view.
PDB entry 2a6v coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structures of the carbohydrate recognition domain of Ca2+-independent cargo receptors Emp46p and Emp47p.
Satoh T, Sato K, Kanoh A, Yamashita K, Yamada Y, Igarashi N, Kato R, Nakano A, Wakatsuki S
J Biol Chem 281 10410-9 (2006)
PMID: 16439369

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171352 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein EMP46 Chains: A, B
Molecule details ›
Chains: A, B
Length: 226 amino acids
Theoretical weight: 25.59 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12396 (Residues: 52-275; Coverage: 56%)
Gene names: EMP46, YLR080W
Sequence domains: Legume-like lectin family
Structure domains: Jelly Rolls

Ligands and Environments

2 bound ligands:
Ligand K 2 x K
Ligand EDO 3 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-18B, PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P21
Unit cell:
a: 54.32Å b: 55.89Å c: 77.47Å
α: 90° β: 108.28° γ: 90°
R-values:
R R work R free
0.191 0.189 0.218
Expression system: Escherichia coli

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Citations

3 review citations

The roles of direct recognition by animal lectins in antiviral immunity and viral pathogenesis.
Liu et al. (2015)
2 more