› 2a7g

X-ray diffraction
1.85Å resolution

On the Routine Use of Soft X-Rays in Macromolecular Crystallography, Part III- The Optimal Data Collection Wavelength

Released:
DOI: 10.2210/pdb2a7g/pdb
PDB entry 2a7g coloured by chain, front view.
PDB entry 2a7g coloured by chain, side view.
PDB entry 2a7g coloured by chain, top view.
Source organism: Bacillus thermoproteolyticus
Primary publication:
On the routine use of soft X-rays in macromolecular crystallography. Part III. The optimal data-collection wavelength.
Mueller-Dieckmann C, Panjikar S, Tucker PA, Weiss MS
Acta Crystallogr D Biol Crystallogr 61 1263-72 (2005)
PMID: 16131760

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: -|-Leu > -|-Phe.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133580 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thermolysin Chain: E
Molecule details ›
Chain: E
Length: 316 amino acids
Theoretical weight: 34.36 KDa
Source organism: Bacillus thermoproteolyticus
UniProt:
  • Canonical: P00800 (Residues: 233-548; Coverage: 61%)
Gene name: npr
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand ZN 1 x ZN
Ligand CA 6 x CA
Ligand DMS 2 x DMS
Ligand ACY 1 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P6122
Unit cell:
a: 92.99Å b: 92.99Å c: 130.21Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.162 0.161 0.189

Quick links

Citations

2 review citations

SAD phasing: History, current impact and future opportunities.
Rose et al. (2016)
1 more