› 2af4

X-ray diffraction
2.15Å resolution

Phosphotransacetylase from Methanosarcina thermophila co-crystallized with coenzyme A

Released:
DOI: 10.2210/pdb2af4/pdb
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PDB entry 2af4 coloured by chain, top view.
Source organism: Methanosarcina thermophila
Primary publication:
Structural and functional studies suggest a catalytic mechanism for the phosphotransacetylase from Methanosarcina thermophila.
Lawrence SH, Luther KB, Schindelin H, Ferry JG
J Bacteriol 188 1143-54 (2006)
PMID: 16428418

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + phosphate = CoA + acetyl phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153661 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphate acetyltransferase Chains: C, D
Molecule details ›
Chains: C, D
Length: 333 amino acids
Theoretical weight: 35.27 KDa
Source organism: Methanosarcina thermophila
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P38503 (Residues: 1-333; Coverage: 100%)
Gene name: pta
Sequence domains: Phosphate acetyl/butaryl transferase
Structure domains:

Ligands and Environments


Cofactor: Ligand COA 2 x COA
No bound ligands
1 modified residue:
Ligand CSO 2 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: I41
Unit cell:
a: 116.488Å b: 116.488Å c: 127.46Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.203 0.272
Expression system: Escherichia coli BL21(DE3)

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Citations

5 review citations

How to make a living by exhaling methane.
Ferry JG. (2010)
4 more

5 mentions without citation

Ancient Regulatory Role of Lysine Acetylation in Central Metabolism.
Nakayasu et al. (2017)
4 more

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