› 2amm

X-ray diffraction
1.9Å resolution

Crystal structure of L122V/L132V mutant of nitrophorin 2

Released:
DOI: 10.2210/pdb2amm/pdb
PDB entry 2amm coloured by chain, front view.
PDB entry 2amm coloured by chain, side view.
PDB entry 2amm coloured by chain, top view.
Source organism: Rhodnius prolixus
Entry authors: Weichsel A, Berry RE, Walker FA, Montfort WR

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173241 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrophorin-2 Chain: X
Molecule details ›
Chain: X
Length: 180 amino acids
Theoretical weight: 20.05 KDa
Source organism: Rhodnius prolixus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q26241 (Residues: 24-202; Coverage: 100%)
Sequence domains: Nitrophorin
Structure domains: Lipocalin

Ligands and Environments

1 bound ligand:
Ligand HEM 1 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-ID-B
Spacegroup: P41212
Unit cell:
a: 34.147Å b: 34.147Å c: 256.223Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.146 0.142 0.221
Expression system: Escherichia coli BL21(DE3)

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