› 2b73

X-ray diffraction
2.15Å resolution

T4 Lysozyme mutant L99A at 100 MPa

Released:
DOI: 10.2210/pdb2b73/pdb
PDB entry 2b73 coloured by chain, front view.
PDB entry 2b73 coloured by chain, side view.
PDB entry 2b73 coloured by chain, top view.
Source organism: Escherichia virus T4
Primary publication:
Cooperative water filling of a nonpolar protein cavity observed by high-pressure crystallography and simulation.
Collins MD, Hummer G, Quillin ML, Matthews BW, Gruner SM
Proc Natl Acad Sci U S A 102 16668-71 (2005)
PMID: 16269539

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133020 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endolysin Chain: A
Molecule details ›
Chain: A
Length: 162 amino acids
Theoretical weight: 18.36 KDa
Source organism: Escherichia virus T4
Expression system: Escherichia coli
UniProt:
  • Canonical: P00720 (Residues: 1-162; Coverage: 99%)
Gene name: E
Sequence domains: Phage lysozyme
Structure domains: Lysozyme

Ligands and Environments

2 bound ligands:
Ligand CL 2 x CL
Ligand BME 2 x BME
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P3221
Unit cell:
a: 60.768Å b: 60.768Å c: 96.077Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.168 0.166 0.219
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Water in nonpolar confinement: from nanotubes to proteins and beyond.
Rasaiah et al. (2008)
13 more

2 mentions without citation

Cooperative water filling of a nonpolar protein cavity observed by high-pressure crystallography and simulation.
Collins et al. (2005)
1 more