› 2cbz

X-ray diffraction
1.5Å resolution

Structure of the human Multidrug Resistance Protein 1 Nucleotide Binding Domain 1

Released:
DOI: 10.2210/pdb2cbz/pdb
PDB entry 2cbz coloured by chain, front view.
PDB entry 2cbz coloured by chain, side view.
PDB entry 2cbz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the human multidrug resistance protein 1 nucleotide binding domain 1 bound to Mg2+/ATP reveals a non-productive catalytic site.
Ramaen O, Leulliot N, Sizun C, Ulryck N, Pamlard O, Lallemand JY, Tilbeurgh Hv, Jacquet E
J Mol Biol 359 940-9 (2006)
PMID: 16697012

Function and Biology Details

Reactions catalysed: 
ATP + H(2)O + xenobiotic(Side 1) = ADP + phosphate + xenobiotic(Side 2)
ATP + H(2)O + glutathione-S-conjugate(Side 1) = ADP + phosphate + glutathione-S-conjugate(Side 2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-543036 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Multidrug resistance-associated protein 1 Chain: A
Molecule details ›
Chain: A
Length: 237 amino acids
Theoretical weight: 25.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P33527 (Residues: 642-871; Coverage: 15%)
Gene names: ABCC1, MRP, MRP1
Sequence domains: ABC transporter
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand ATP 1 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P31
Unit cell:
a: 65.56Å b: 65.56Å c: 64.612Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.161 0.16 0.187
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

Review. SUR1: a unique ATP-binding cassette protein that functions as an ion channel regulator.
Aittoniemi et al. (2009)
10 more

29 mentions without citation

Cellular Models and In Vitro Assays for the Screening of modulators of P-gp, MRP1 and BCRP.
Gameiro et al. (2017)
28 more