› 2cvz

X-ray diffraction
1.8Å resolution

Structure of hydroxyisobutyrate dehydrogenase from thermus thermophilus HB8

Released:
DOI: 10.2210/pdb2cvz/pdb
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Source organism: Thermus thermophilus HB8
Primary publication:
Crystal structure of novel NADP-dependent 3-hydroxyisobutyrate dehydrogenase from Thermus thermophilus HB8.
Lokanath NK, Ohshima N, Takio K, Shiromizu I, Kuroishi C, Okazaki N, Kuramitsu S, Yokoyama S, Miyano M, Kunishima N
J Mol Biol 352 905-17 (2005)
PMID: 16126223

Function and Biology Details

Reaction catalysed: 
3-hydroxy-2-methylpropanoate + NAD(+) = 2-methyl-3-oxopropanoate + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178204 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-hydroxyisobutyrate dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 289 amino acids
Theoretical weight: 31.66 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SLQ6 (Residues: 1-289; Coverage: 100%)
Gene name: TTHA0237
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NDP 3 x NDP
No bound ligands
1 modified residue:
Ligand MSE 24 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P212121
Unit cell:
a: 85.878Å b: 106.367Å c: 168.639Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.182 0.2
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

Evolution of protein fold in the presence of functional constraints.
Andreeva et al. (2006)
2 more

3 mentions without citation

NAD(P)H-Dependent Dehydrogenases for the Asymmetric Reductive Amination of Ketones: Structure, Mechanism, Evolution and Application.
Sharma et al. (2017)
2 more

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