› 2dhf

X-ray diffraction
2.3Å resolution

CRYSTAL STRUCTURES OF RECOMBINANT HUMAN DIHYDROFOLATE REDUCTASE COMPLEXED WITH FOLATE AND 5-DEAZOFOLATE

Released:
DOI: 10.2210/pdb2dhf/pdb
PDB entry 2dhf coloured by chain, front view.
PDB entry 2dhf coloured by chain, side view.
PDB entry 2dhf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structures of recombinant human dihydrofolate reductase complexed with folate and 5-deazafolate.
Davies JF, Delcamp TJ, Prendergast NJ, Ashford VA, Freisheim JH, Kraut J
Biochemistry 29 9467-79 (1990)
PMID: 2248959

Function and Biology Details

Reaction catalysed: 
5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132448 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydrofolate reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 186 amino acids
Theoretical weight: 21.35 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P00374 (Residues: 2-187; Coverage: 100%)
Gene name: DHFR
Sequence domains: Dihydrofolate reductase
Structure domains: Dihydrofolate Reductase, subunit A

Ligands and Environments

1 bound ligand:
Ligand DZF 2 x DZF
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P1
Unit cell:
a: 36.1Å b: 38.5Å c: 76.8Å
α: 93.9° β: 91.4° γ: 111.5°
R-values:
R R work R free
0.194 not available not available
Expression system: Not provided

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Citations

10 review citations

High-dose vitamin therapy stimulates variant enzymes with decreased coenzyme binding affinity (increased K(m)): relevance to genetic disease and polymorphisms.
Ames et al. (2002)
9 more

11 mentions without citation

Database of traditional Chinese medicine and its application to studies of mechanism and to prescription validation.
Chen et al. (2006)
10 more