› 2e7l

X-ray diffraction
2.5Å resolution

Structure of a high-affinity mutant of the 2C TCR in complex with Ld/QL9

Released:
DOI: 10.2210/pdb2e7l/pdb
PDB entry 2e7l coloured by chain, front view.
PDB entry 2e7l coloured by chain, side view.
PDB entry 2e7l coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
How a single T cell receptor recognizes both self and foreign MHC.
Colf LA, Bankovich AJ, Hanick NA, Bowerman NA, Jones LL, Kranz DM, Garcia KC
Cell 129 135-46 (2007)
PMID: 17418792

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-106898 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Ig-like domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 113 amino acids
Theoretical weight: 12.7 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A2NTU7 (Residues: 21-132; Coverage: 45%)
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
Ig-like domain-containing protein Chains: C, D
Molecule details ›
Chains: C, D
Length: 121 amino acids
Theoretical weight: 13.27 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A2NTY6 (Residues: 30-144; Coverage: 96%)
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
H-2 class I histocompatibility antigen, L-D alpha chain Chains: E, F
Molecule details ›
Chains: E, F
Length: 181 amino acids
Theoretical weight: 21.21 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P01897 (Residues: 25-205; Coverage: 54%)
Gene name: H2-L
Sequence domains: Class I Histocompatibility antigen, domains alpha 1 and 2
Structure domains: MHC class I-like antigen recognition-like
Peptide (GLN)(LEU)(SER)(PRO)(PHE)(PRO)(PHE)(ASP)(LEU) Chains: P, Q
Molecule details ›
Chains: P, Q
Length: 9 amino acids
Theoretical weight: 1.06 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P42212
Unit cell:
a: 113.54Å b: 113.54Å c: 177.452Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.224 0.246
Expression system: Escherichia coli BL21

Quick links

Citations

57 review citations

T cell antigen receptor recognition of antigen-presenting molecules.
Rossjohn et al. (2015)
56 more

13 mentions without citation

Thermodynamics of T-cell receptor-peptide/MHC interactions: progress and opportunities.
Armstrong et al. (2008)
12 more