› 2ec5

X-ray diffraction
2.6Å resolution

Crystal structures reveal a thiol-protease like catalytic triad in the C-terminal region of Pasteurella multocida toxin

Released:
DOI: 10.2210/pdb2ec5/pdb
PDB entry 2ec5 coloured by chain, front view.
PDB entry 2ec5 coloured by chain, side view.
PDB entry 2ec5 coloured by chain, top view.
Source organism: Pasteurella multocida
Primary publication:
Crystal structures reveal a thiol protease-like catalytic triad in the C-terminal region of Pasteurella multocida toxin.
Kitadokoro K, Kamitani S, Miyazawa M, Hanajima-Ozawa M, Fukui A, Miyake M, Horiguchi Y
Proc Natl Acad Sci U S A 104 5139-44 (2007)
PMID: 17360394

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148069 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dermonecrotic toxin Chains: A, B
Molecule details ›
Chains: A, B
Length: 746 amino acids
Theoretical weight: 84.28 KDa
Source organism: Pasteurella multocida
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P17452 (Residues: 569-1285; Coverage: 56%)
Gene name: toxA
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: C2
Unit cell:
a: 197.357Å b: 132.823Å c: 82.546Å
α: 90° β: 114.74° γ: 90°
R-values:
R R work R free
0.235 0.232 0.286
Expression system: Escherichia coli BL21

Quick links

Citations

13 review citations

Structure and mode of action of clostridial glucosylating toxins: the ABCD model.
Jank et al. (2008)
12 more

5 mentions without citation

Recent insights into Pasteurella multocida toxin and other G-protein-modulating bacterial toxins.
Wilson et al. (2010)
4 more