› 2gb8

Solution NMR

Solution structure of the complex between yeast iso-1-cytochrome c and yeast cytochrome c peroxidase

Released:
DOI: 10.2210/pdb2gb8/pdb
PDB entry 2gb8 coloured by chain, ensemble of 20 models, front view.
PDB entry 2gb8 coloured by chain, ensemble of 20 models, side view.
PDB entry 2gb8 coloured by chain, ensemble of 20 models, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Solution structure and dynamics of the complex between cytochrome c and cytochrome c peroxidase determined by paramagnetic NMR.
Volkov AN, Worrall JA, Holtzmann E, Ubbink M
Proc Natl Acad Sci U S A 103 18945-50 (2006)
PMID: 17146057

Function and Biology Details

Reaction catalysed: 
2 ferrocytochrome c + H(2)O(2) = 2 ferricytochrome c + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132231 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cytochrome c peroxidase, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 294 amino acids
Theoretical weight: 33.53 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P00431 (Residues: 68-361; Coverage: 81%)
Gene names: CCP, CCP1, CPO, YKR066C
Sequence domains: Peroxidase
Structure domains:
Cytochrome c isoform 1 Chain: B
Molecule details ›
Chain: B
Length: 108 amino acids
Theoretical weight: 12.07 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P00044 (Residues: 2-109; Coverage: 99%)
Gene names: CYC1, J1653, YJR048W
Sequence domains: Cytochrome c
Structure domains: Cytochrome c-like domain

Ligands and Environments


Cofactor: Ligand HEC 1 x HEC

Cofactor: Ligand HEM 1 x HEM
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: rigid-body docking solely on the basis of experimental data; sidechain dynamics.
Expression system: Escherichia coli BL21

Quick links

Citations

34 review citations

Reaching for high-hanging fruit in drug discovery at protein-protein interfaces.
Wells et al. (2007)
33 more

3 mentions without citation

Solution structure and dynamics of the complex between cytochrome c and cytochrome c peroxidase determined by paramagnetic NMR.
Volkov et al. (2006)
2 more