› 2gx2

X-ray diffraction
1.8Å resolution

Crystal structural and functional analysis of GFP-like fluorescent protein Dronpa

Released:
DOI: 10.2210/pdb2gx2/pdb
PDB entry 2gx2 coloured by chain, front view.
PDB entry 2gx2 coloured by chain, side view.
PDB entry 2gx2 coloured by chain, top view.
Source organism: Echinophyllia sp. SC22
Primary publication:
Structural characterization of the photoswitchable fluorescent protein Dronpa-C62S.
Nam KH, Kwon OY, Sugiyama K, Lee WH, Kim YK, Song HK, Kim EE, Park SY, Jeon H, Hwang KY
Biochem Biophys Res Commun 354 962-7 (2007)
PMID: 17276392

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178339 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fluorescent protein Dronpa Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 241 amino acids
Theoretical weight: 27.6 KDa
Source organism: Echinophyllia sp. SC22
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5TLG6 (Residues: 2-224; Coverage: 100%)
Gene name: Dronpa
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand MG 62 x MG
1 modified residue:
Ligand GYS 12 x GYS

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 4A, PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P1
Unit cell:
a: 73.091Å b: 106.16Å c: 110.07Å
α: 61.17° β: 70.85° γ: 86.21°
R-values:
R R work R free
0.182 0.182 0.209
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 citation in other articles

Light-dependent regulation of structural flexibility in a photochromic fluorescent protein.
Mizuno et al. (2008)
8 more

4 mentions without citation

X-ray Free Electron Laser Determination of Crystal Structures of Dark and Light States of a Reversibly Photoswitching Fluorescent Protein at Room Temperature.
Hutchison et al. (2017)
3 more