› 2i94

Solution NMR

NMR Structure of recoverin bound to rhodopsin kinase

Released:
DOI: 10.2210/pdb2i94/pdb
PDB entry 2i94 coloured by chain, ensemble of 10 models, front view.
PDB entry 2i94 coloured by chain, ensemble of 10 models, side view.
PDB entry 2i94 coloured by chain, ensemble of 10 models, top view.
Source organism: Bos taurus
Primary publication:
Structural basis for calcium-induced inhibition of rhodopsin kinase by recoverin.
Ames JB, Levay K, Wingard JN, Lusin JD, Slepak VZ
J Biol Chem 281 37237-45 (2006)
PMID: 17020884

Function and Biology Details

Reaction catalysed: 
ATP + [rhodopsin] = ADP + [rhodopsin] phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-539778 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Recoverin Chain: A
Molecule details ›
Chain: A
Length: 202 amino acids
Theoretical weight: 23.37 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P21457 (Residues: 1-202; Coverage: 100%)
Gene names: RCV1, RCVRN
Sequence domains: EF hand
Structure domains: EF-hand
Rhodopsin kinase GRK1 Chain: B
Molecule details ›
Chain: B
Length: 25 amino acids
Theoretical weight: 2.56 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P28327 (Residues: 1-25; Coverage: 5%)
Gene names: GRK1, RHOK

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing molecular dynamics
Expression system: Escherichia coli BL21(DE3)

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Citations

14 review citations

Neuronal calcium sensor proteins: generating diversity in neuronal Ca2+ signalling.
Burgoyne RD. (2007)
13 more

11 mentions without citation

Understanding the physiological roles of the neuronal calcium sensor proteins.
Burgoyne et al. (2012)
10 more