› 2iv0

X-ray diffraction
2.5Å resolution

Thermal stability of isocitrate dehydrogenase from Archaeoglobus fulgidus studied by crystal structure analysis and engineering of chimers

Released:
DOI: 10.2210/pdb2iv0/pdb
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Source organism: Archaeoglobus fulgidus
Primary publication:
Thermal stability of isocitrate dehydrogenase from Archaeoglobus fulgidus studied by crystal structure analysis and engineering of chimers.
Stokke R, Karlström M, Yang N, Leiros I, Ladenstein R, Birkeland NK, Steen IH
Extremophiles 11 481-93 (2007)
PMID: 17401542

Function and Biology Details

Reaction catalysed: 
(1a) isocitrate + NADP(+) = 2-oxalosuccinate + NADPH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128149 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Isocitrate dehydrogenase [NADP] Chains: A, B
Molecule details ›
Chains: A, B
Length: 412 amino acids
Theoretical weight: 45.9 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli
UniProt:
  • Canonical: O29610 (Residues: 1-412; Coverage: 100%)
Gene names: AF_0647, icd
Sequence domains: Isocitrate/isopropylmalate dehydrogenase
Structure domains: Isopropylmalate Dehydrogenase

Ligands and Environments

2 bound ligands:
Ligand ZN 9 x ZN
Ligand CL 3 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 81.611Å b: 65.405Å c: 87.181Å
α: 90° β: 95.28° γ: 90°
R-values:
R R work R free
0.199 0.196 0.254
Expression system: Escherichia coli

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Citations

12 citation in other articles

Structural and functional properties of isocitrate dehydrogenase from the psychrophilic bacterium Desulfotalea psychrophila reveal a cold-active enzyme with an unusual high thermal stability.
Fedøy et al. (2007)
11 more

2 mentions without citation

Dimeric interactions and complex formation using direct coevolutionary couplings.
dos Santos et al. (2015)
1 more

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